FastQCFastQC Report
Wed 25 May 2016
SRR1700019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3542098
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65400.18463633699575788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46550.1314192887943812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.027.135541
GTACCTG30600.024.9481431
TACCTGG30900.024.1031572
ATAACGC2650.023.703173
GTATCAA87900.022.7343141
TATAACG2700.022.4333612
TAACGCA3200.022.4320934
CAACGCG1356.730261E-1122.0206186
ACCTGGG35100.020.4520653
AACGCGG1659.931682E-1018.0168697
ACGTCCG1802.1645974E-1017.44718443
ATCAACG114300.017.1537693
TCAACGC115100.016.99464
CAACGCA114950.016.9777495
TATCAAC118350.016.7563082
CCTGGGG24400.016.456334
AACGCAG117950.016.2855056
ACGCGGA1906.4683263E-915.6462298
GTACCGG2405.2750693E-1114.9688861
GTCTCGC5250.014.9688851
Produced by FastQC (version 0.11.5)