FastQCFastQC Report
Wed 25 May 2016
SRR1700018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5330342
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA104490.19602869759576402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86830.16289761519992527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86100.16152809707144494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60990.11442042555618381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG2800.018.3935596
AACGCGG3100.016.6135377
GGTATCA143050.016.2873061
ACGCGGA3650.014.1101278
AGCGTAC2307.1559043E-913.63000810
TCGCGCA3500.013.43516917
GTATCAA200500.013.43055251
AATCGCG3700.013.31413215
CGATCGT2055.101976E-612.01519114
GGACCGT3001.879016E-911.9424856
TACACTG16950.011.7576455
TTTCGCG2301.4385205E-611.6828649
GCGGAGT4600.011.68286410
TATACCG2158.670791E-611.456565
TTCGCGA2351.8637147E-611.43429310
ATAATAC17050.011.4264473
CGGAGTA3959.094947E-1211.33769511
TCTATAC12650.011.32937053
CAACGCT3401.1186785E-911.1960796
TAATACA24250.011.1736234
Produced by FastQC (version 0.11.5)