FastQCFastQC Report
Wed 25 May 2016
SRR1700017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9105837
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA456870.5017331190971243No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA194540.21364318293859205No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT148360.16292846006358339No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT138830.15246264566343545No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC130160.1429412804116744No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA113060.12416211711235331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109460.12020860904933836No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA94180.10342816371520817No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT93850.10306575880943179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA2450.019.19449625
CAACGCG4300.015.6227416
ACGATCG2700.015.40046420
GTATTAG25350.013.6999251
ACGCGGA5250.013.648828
AGCGTAC3550.013.24632410
GTACATG238000.013.1894181
AACGCGG5650.013.078867
TCTATAC20550.012.9677143
GAGTACT186550.012.92695512
ATCGCGA3403.6379788E-1212.90957723
ACGCATA4350.012.86930757
AGTACTT192450.012.85642313
AGAGTAC302850.012.84245511
TACATGG234750.012.7256062
TATACTG25250.012.4160375
CGCGTAA1455.053862E-412.35453510
TACACCG4950.012.2144785
CGCATAG4400.012.2141428
ACATGGG239650.011.97016053
Produced by FastQC (version 0.11.5)