FastQCFastQC Report
Wed 25 May 2016
SRR1700016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5188778
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA101260.19515192209032647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66220.1276215710134448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA180900.015.1400231
TCGACGC1209.76169E-515.0001773
CAGCGTA2601.0913936E-1114.7102959
ACGAACG1453.110127E-513.96473929
AGCGTAC2103.0000592E-813.92739310
TCGCGCA2451.1004886E-913.77434617
CGAACGC1656.9266534E-613.6352130
ATCGCGA1352.6516817E-413.33516123
CGCCGTA2907.2759576E-1113.19095425
TACCGCG2053.3738797E-713.1696188
AGAGTAC185400.013.16636111
GTACATG123750.013.0498481
AACGCGG1902.0672542E-613.0252146
GGTATCA120950.012.9981351
CGTAGCG1403.6128404E-412.85605515
GAGTACT129850.012.7001212
AGTACTT131300.012.54273213
ATCAACG214850.012.5146923
TCAACGC215050.012.5129134
TACATGG123000.012.4757572
Produced by FastQC (version 0.11.5)