FastQCFastQC Report
Wed 25 May 2016
SRR1700015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6565848
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111130.16925460351808327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74060.11279578814495858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81050.022.8645571
GTATCAA163400.022.2438071
TAACGCA2800.018.39514
ATCAACG205450.017.3191663
TCAACGC207500.017.1705594
TATCAAC210400.016.9755612
CAACGCA210400.016.9117035
CGCGCAA2351.8189894E-1216.70976818
AACGCAG211900.016.2843696
GTACCTG37950.015.7065271
TACCTGG36500.015.4602622
CGTATCG1451.9046674E-615.44298610
CAACGCG2202.3464963E-1015.2674986
CGCGAAA1502.7073693E-614.9294725
TATAACG4100.014.7465052
ACGCAGA240650.014.3202977
CGCAGAG244500.014.0673298
GTACATG160100.014.0524711
AGCGTAC2408.6765795E-1013.99520610
AGAGTAC234300.013.87619811
Produced by FastQC (version 0.11.5)