FastQCFastQC Report
Wed 25 May 2016
SRR1700014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035785
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19630.18951809497144678No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA18310.1767741374899231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14190.1369975429263795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.10069657313052419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG450.001227040524.9980166
AACGCGG654.2401126E-420.7675827
TGGTACG600.00651305618.74851241
TAACGCA850.00256464115.8826264
ACTGTCC2302.5465852E-1115.6509328
TACCTGG6850.015.1104772
GTACCTG7150.014.7976071
GTATCAA31450.014.4587381
GTATAAA4550.014.3478321
GTACATG21200.013.9102551
GTCTATA1951.8171158E-713.853081
GACTGTC2652.4374458E-1013.5838287
TCTATAC2551.9881554E-913.236163
TACATGG20750.013.2297812
CTATACC2053.3609285E-713.17095854
ACCTGGG7450.012.9874273
ACATGGG20150.012.9537013
GGTTAGA1403.590721E-412.8635751
GGTATCA21850.012.7752661
AGAGTAC32000.012.65524511
Produced by FastQC (version 0.11.5)