FastQCFastQC Report
Wed 25 May 2016
SRR1700013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888108
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA166170.5753593702174572No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA72320.25040614824653373No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT55820.19327532072900322No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48940.1694534968913905No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC47260.1636365399077874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46360.16052031295228572No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT43850.15182950222083108No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA36850.12759218145581813No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA31040.10747520522085738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG1151.2732926E-1124.81592220
GTACCTG25000.021.9692841
ATCGCGA1307.094059E-1121.95368623
ATAACGC2100.021.9479853
TACCTGG24600.021.6803282
CGCGAAA1351.1641532E-1021.13948825
CCGATCG856.2448926E-620.6569279
TCGCGAA1605.4569682E-1119.20947524
TCCGACG959.215265E-519.18897631
CGCGCAA2350.018.68107818
ACCTGGG28000.018.6557873
TATAACG2600.017.7272192
TCGCGCA2550.017.21517
AGCGTCG1551.483204E-816.9928740
GCAATCG2900.016.65019413
AATCGCG2900.016.65019415
CGACGCC1102.829624E-416.57229833
TAACGCA2400.016.4609874
GCGCAAC2850.016.17443819
CCTCGCG953.4092518E-416.17229
Produced by FastQC (version 0.11.5)