FastQCFastQC Report
Wed 25 May 2016
SRR1700012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775242
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15920.2053552310117357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13880.17904086723887508No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA9580.12357431614902187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG458.698571E-734.9981965
GTACCTG8450.025.8587591
TACCTGG8550.024.475582
ACCTGGG8700.024.310663
AGGGCCG855.03097E-621.1753775
CGTTCCG654.2379918E-420.76815834
CGCGTTC654.2379918E-420.76815832
GGTATCA17550.020.0235061
GCGCGTT706.9830887E-419.28471831
CACGCGC750.001110164217.99907328
GCGTTCC901.8654241E-417.49909833
GTCCTAC1305.7059333E-717.3280331
CCTACAC1701.8262654E-917.2061043
GCACGCG800.001710757316.8741327
GTTGCCG800.001710757316.8741340
TTATACC1752.6866473E-916.7134234
CCTGGGG7000.016.7134234
GTCTCGC952.7927323E-416.5984331
GGTTATA952.7927323E-416.5984331
ACACCGT952.8189793E-416.5780936
Produced by FastQC (version 0.11.5)