FastQCFastQC Report
Wed 25 May 2016
SRR1700011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822779
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA126130.4468291708277552No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA58200.20617979657635258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57950.20529414452920333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49040.17372950556880296No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT39680.14057069292353386No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34540.12236168683414465No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT33670.11927961771006515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.10089348121124608No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA28460.10082262904747413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG42050.031.2240031
TACCTGG42250.030.6747862
TATAACG2800.028.125212
ACCTGGG47300.026.8284783
ATAACGC3350.024.1788583
CCTGGGG31900.023.556924
GTATAAC5750.021.9334131
TAACGCA3700.021.8908924
CGCGAAA908.252651E-620.00156825
GTACCGG2600.019.0561581
GTACCCG3250.018.7096821
ACGATCG851.20483324E-418.53020720
GTCTCGC5750.017.6250631
TCTGTCG6900.017.6082048
GACGCTA1459.68721E-817.06818640
TATCACG2550.016.7648322
TACCCGG3500.016.714412
GTACACG2700.016.6821581
CTGGGGG18250.016.5197945
TCGCTCT6650.016.239864
Produced by FastQC (version 0.11.5)