FastQCFastQC Report
Wed 25 May 2016
SRR1700010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11002517
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119010.10816615870713946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132700.026.5646211
GTATCAA251500.021.548541
CGCGAAA7400.017.02825725
ATCAACG329850.016.459823
TCAACGC333000.016.3707944
TATCAAC333850.016.054122
ACGATCG7150.016.05003720
ATCGCGA7450.016.00808523
CAACGCA353650.015.4148865
AACGCAG360600.015.292496
TCGCGAA8400.014.46539624
CGCGCAA17950.014.28916718
ACGCAGA405300.013.555947
GCGCAAC19350.013.48787519
ATCGCGC19100.013.42870116
TCGCGCA18450.013.292191517
CGCAACG19650.013.28334120
AACGATA20000.013.2761423
CGCAGAG416400.013.17836958
GTCGGTA9400.013.16422943
Produced by FastQC (version 0.11.5)