FastQCFastQC Report
Wed 25 May 2016
SRR1700009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5127131
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA56670.1105296509880477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111400.017.2980671
ATCGACG902.1589725E-417.1707632
CGACGCT5350.014.85563716
GCTCCGA4250.014.76696129
CGCAACG7700.014.62479420
TACCGAC5750.014.20615113
TCGCACG1109.921929E-414.04908118
CCGATCG1109.921929E-414.04908114
ACGTCCG1901.6833292E-713.94317743
TCGCGCA7500.013.83517
ACCGACG5800.013.70304314
CCGACGC5800.013.70304315
GCTACCG6250.013.06940411
CGTTCCG7300.013.00420834
CGCGCAA8150.013.002480518
CGATCGT6050.012.77164236
GCGCAAC8650.012.76296219
TCGCCCC4350.012.68775638
TTCGCGA5750.012.66106532
CAACGCG1404.237777E-412.6152545
Produced by FastQC (version 0.11.5)