FastQCFastQC Report
Wed 25 May 2016
SRR1700008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8876979
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131400.020.7079771
GTATCAA216800.017.4102551
CGCAACG8300.016.74538820
CGCGCAA8800.015.79261118
GCGCAAC9200.015.34653219
GTACCTG53100.014.9737421
TCGCGCA9150.014.94743417
TACCTGG52850.014.399932
AATCGCG9400.014.31522215
TATACCG3350.013.8280455
AACGATA9600.013.78883323
ATCAACG274350.013.580353
TCAACGC275750.013.55944
TATCAAC282400.013.2557232
TATACTG32650.013.1746085
ATCGCGC10400.013.15086816
GCAACGA10600.013.11239821
CAACGCA285900.012.9468495
TATAACG4800.012.86776352
AACGCAG292850.012.8429696
Produced by FastQC (version 0.11.5)