FastQCFastQC Report
Wed 25 May 2016
SRR1700007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240038
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.1041053767837867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2950.024.7365824
GTACCTG24400.024.3844031
ATAACGC3000.024.3243063
TACCTGG24000.024.1454522
TATAACG2850.024.0983832
TATCACG1850.020.8825532
GTACCCG2450.020.1643371
ACCTGGG28800.019.8231163
GGTATCA46900.019.1894871
CCTGGGG19750.018.6914984
TCACGCA2400.016.0969684
GTATAAC4550.016.0505431
TACCCGG2750.015.6091812
CGCGCAA1251.1938138E-515.45377818
GTTATAC3350.014.7355333
GTACCGG2103.5706762E-914.3196021
TATACCG1504.2043685E-614.3084165
TATACTG7700.014.2155035
CTGGGGG11200.014.1806625
ATCTCGG13100.014.0747937
Produced by FastQC (version 0.11.5)