FastQCFastQC Report
Wed 25 May 2016
SRR1700005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5072808
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76600.025.0592481
AATCCGC9200.018.4299186
GCTACCG8950.018.26812211
CGACGCT8950.017.26060916
TACCGAC9600.016.3154213
CCGACGC9500.016.2613115
GTATCAA120100.015.8816952
TCCGCTA11200.015.4091978
CGCTACC11100.015.27524710
CGCGCAA10000.015.23383518
ACCGACG10150.015.21994714
CGTTCCG8550.015.05662134
TCGCGAT10400.014.85408333
CTACCGA11350.014.67202412
TTTCGCG10400.014.5403631
CGATCGT10050.014.51727936
TTCGCGA10300.014.47179132
AACGATA10900.014.37053623
ATCCGCT11850.014.3084597
GCGCAAC10650.014.30421219
Produced by FastQC (version 0.11.5)