FastQCFastQC Report
Wed 25 May 2016
SRR1700004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6807134
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87450.021.9321751
GTATCAA147200.017.7809931
GTACCTG36700.015.374241
TACCTGG37000.014.8886592
CGCGCAA5600.013.77986718
AACGCAG183550.013.7684055
CGCAACG5800.013.30479720
ACCTGGG40850.013.2230823
ATACCGT4650.013.0965116
TCGCGTA1400.00945374913.0497389
TCGCGCA5750.013.04760417
ACGCAGA194550.012.9435337
CAACGCA196750.012.8337845
CGCAGAG195800.012.8297988
ATCAACG200500.012.7220413
AGAGTAC186150.012.70973711
TCAACGC201400.012.665194
TATCAAC205300.012.4663572
CGACGCT6750.012.38486916
ATCGCGC6250.012.34676116
Produced by FastQC (version 0.11.5)