FastQCFastQC Report
Wed 25 May 2016
SRR1700003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8345562
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN121400.1454665365855529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN91670.10984281226357195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88850.023.7226921
GTATCAA173150.020.8735141
GTACCTG67900.020.4677011
TACCTGG68850.019.4588762
ACCTGGG74650.017.3869743
CGCGAAA3100.017.03154225
ATCAACG211550.016.7214833
TCAACGC212550.016.6324634
CAACGCA219450.016.14965
TATCAAC221650.016.0886542
ATAACGC4700.015.9141293
AACGCAG227100.015.6540246
ATCGCGA3350.014.4472423
TATAACG5600.014.1422622
ACGATCG3300.014.00041820
ACGCAGA260050.013.6536427
TAACGCA5200.013.537814
CGCAGAG264100.013.4192718
TATCACG6600.013.3327712
GAGTACT159300.013.20236512
Produced by FastQC (version 0.11.5)