FastQCFastQC Report
Wed 25 May 2016
SRR1700002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10432857
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN215540.20659729161436796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN184650.17698891109118048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN140280.1344598128777189No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN110050.1054840490960434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG106850.023.3820741
TACCTGG105700.023.0821442
ACCTGGG118100.020.0802783
TATAACG7350.019.1563262
TAACGCA7700.018.2847964
ATAACGC8600.018.1618793
CCTGGGG95750.016.0367934
CGCGCAA6850.015.09485518
CGCGAAA4100.015.02370825
ACGATCG3800.014.47372120
GTATCAA353000.014.2611971
TACACGG8500.013.9763712
GTACCCG10450.013.4929891
GTATAAC19600.013.3762811
GGTATCA236450.013.3055671
CGATCGT5700.013.12246241
TCGCGCA8400.013.09533317
AATCGCG7750.013.05803615
TATACTG32150.012.9324765
GTACATG292800.012.5958911
Produced by FastQC (version 0.11.5)