FastQCFastQC Report
Wed 25 May 2016
SRR1700001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7686566
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN342770.44593385394726337No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN182170.23699789997249748No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN121050.1574825481235704No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN111830.14548759485054832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN100730.13104681596437212No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN93820.12205710586495973No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN82590.10744720073957603No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN80590.10484525859792265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA7000.017.28528218
AATCGCG7800.015.79447815
GTACCTG50350.015.0524411
TCGCGCA8200.014.7557317
CGCAACG8400.014.666320
TACCTGG49500.014.6216672
GCGCAAC8400.014.40440219
ATCGCGA2803.6379788E-1214.14250423
CGATCGT6600.013.99946841
AACGATA8800.013.74965823
ATAACGC4200.013.618533
ATCGCGC9050.013.6129216
GCAACGA9250.013.31858621
CGACGCT6700.013.13421
TTTCGCG7150.012.92258636
GTACATG217250.012.9198531
GCAATCG9400.012.87201813
ACCTGGG53850.012.827743
GAGTACT144800.012.64056912
TACCGAC6800.012.617331518
Produced by FastQC (version 0.11.5)