FastQCFastQC Report
Wed 25 May 2016
SRR1700000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6124779
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN97150.1586179680932161No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN85110.1389601159486734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN69160.11291835999307077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG50450.020.9615291
TACCTGG51350.020.049782
ACCTGGG59200.017.168193
TATAACG3350.017.0739022
GTATCAA177150.016.4909341
ACGATCG1857.588824E-915.45905620
GTACCGG5850.015.4408381
CGTTCCG2850.015.43794134
GGTATCA111100.015.3288051
GTACACG5050.014.8330491
TCGCGAA2102.5520421E-914.66628324
ATAACGC4050.014.6660433
GCCGTTA3200.014.43747615
GTCTCGC6000.014.3204361
CGCGCAA3850.014.28510718
TACACGG4850.014.0612572
TAACGCA3600.014.0549584
TCGCGCA4250.013.97644717
GCGCGTT3150.013.9676631
TACCGCG3400.013.5876588
Produced by FastQC (version 0.11.5)