FastQCFastQC Report
Wed 25 May 2016
SRR1699999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9356863
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN157980.16883863747924918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN133940.14314626600816963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN102890.10996206741511552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4850.018.14395120
CGCGAAA4900.017.50974525
ATCGCGA5200.017.3457923
CGCGCAA12700.016.2829518
CGCAACG13250.015.60722220
GTATCAA292500.015.4995231
TCGCGCA13350.015.49039617
AATCGCG13750.015.03976615
GCGCAAC14100.014.9784119
TCGCGAA6050.014.90877924
GGTATCA188900.014.8688391
GCAACGA14300.014.76892321
GTACCTG53100.014.0638431
AACGATA15500.014.05131923
ATCGCGC15000.013.78645216
TACCTGG51600.013.6855422
CGTATCG1658.7328335E-613.33284110
CGCGTAT1000.00876717813.19979512
CGATCGC6850.013.16760721
GATCGCG6900.013.07219122
Produced by FastQC (version 0.11.5)