FastQCFastQC Report
Wed 25 May 2016
SRR1699998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5790519
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN105480.1821598374860699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN73750.12736336760141878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66700.023.0231971
GTACCTG56450.022.9104121
TACCTGG56000.022.6677552
TATAACG4100.021.99992
TAACGCA4100.021.462394
GTATCAA129750.020.9371
ATAACGC4550.020.3067253
ACCTGGG61950.020.02813
CGCGTAT901.0033733E-519.5547912
ATCAACG162600.016.2895073
TCAACGC164050.016.1455274
TATCAAC167150.016.0441992
GTACCCG4700.015.9382191
CGCGAAA1259.456833E-615.83924425
CAACGCA170050.015.6146625
CCTGGGG47800.015.5557434
AACGCAG176600.015.1351786
GTATTAG14250.014.8427741
ACGATCG1351.9581135E-514.6669820
ATCGCGA1402.7614375E-514.14218323
Produced by FastQC (version 0.11.5)