FastQCFastQC Report
Wed 25 May 2016
SRR1699997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6666240
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN127080.1906322004608295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN89140.1337185579877112No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN76430.11465233774961597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG60050.021.2798671
TACCTGG60950.020.6108992
TATAACG3400.020.0593722
CGCGCAA4250.019.15418218
ACCTGGG66100.018.5708353
ATAACGC3950.018.3787333
GTATCAA182750.018.3730621
GGTATCA107450.016.8956431
TCGCGCA5200.015.654860517
AATCGCG5100.015.52901915
TAACGCA4500.015.1547184
TACCCGG5900.014.9156632
ATCAACG225650.014.55536653
TCAACGC226950.014.4913794
TATCAAC228550.014.4777522
CAACGCA233700.014.1198875
CCTGGGG54300.014.0581564
GTACCCG6300.013.9885721
GCGCAAC5700.013.89522119
GCAACGA6050.013.81866321
Produced by FastQC (version 0.11.5)