FastQCFastQC Report
Wed 25 May 2016
SRR1699996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6345464
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN106030.16709573957081783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN78050.12300124939641924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG51200.023.4864791
TACCTGG55050.021.5802122
GGTATCA84550.019.9270521
ACCTGGG60300.019.2633783
GTATCAA153250.019.1856291
ATAACGC3600.016.4997393
CGCGTAT953.3501236E-416.20988712
CGCGCAA4550.015.95667218
GTACCGG5350.015.6431791
TATAACG4100.015.5608532
ATCAACG187550.015.3311373
TCAACGC187650.015.310884
AATCGCG4750.015.28469115
CCTGGGG44900.015.1397154
TATCAAC191650.015.1180662
CAACGCA194000.014.7983865
TAACGCA3750.014.6660894
AACGCAG200100.014.4352136
CGCAACG5100.014.23495820
GTACACG4950.013.7927771
Produced by FastQC (version 0.11.5)