FastQCFastQC Report
Wed 25 May 2016
SRR1699995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8041198
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN232450.2890738419822519No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN114450.14232953845931912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN96910.12051686825768997No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN88450.10999604785257122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA13750.019.20060218
CGCAACG14300.018.76958720
TCGCGCA14200.018.74706617
ACGATCG4100.018.24424720
AATCGCG14650.018.16997315
GCGCAAC14850.017.92626619
CGCGAAA4150.017.4931125
AACGATA15650.017.43066423
GCAACGA15300.017.39902121
ATCGCGA4450.016.80836723
ATCGCGC16300.016.73651316
CGATAAA18500.015.93434725
GCAATCG17500.015.58799413
ACGATAA17600.015.37423924
CAATCGC17800.015.20168514
TCGCGAA4800.015.12425224
CAACGAT19050.014.666944522
CGTGCAA20300.014.30471110
AGGCCGT20300.013.9796056
CGTCGGT5550.013.87318842
Produced by FastQC (version 0.11.5)