FastQCFastQC Report
Wed 25 May 2016
SRR1699994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8079630
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN121150.14994498510451593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA8200.017.70771418
TCGCGCA8600.016.62827917
AACGATA9500.015.74674723
TATAACG4200.015.7142492
GCGCAAC9150.015.62876419
ACGATAA9750.015.34298324
GTACCTG55900.015.1702341
AATCGCG9450.015.13223815
GTATCAA200250.015.10224151
TACCTGG53050.014.9707472
ATCGCGA2850.014.66608723
ATCGCGC10100.014.37655916
CGCAACG10050.014.22873320
ACGATCG3051.8189894E-1213.70480120
GCAACGA10700.013.56989221
GTACATG242900.013.5114551
TAGACCG2003.2829848E-713.1994785
ACCTGGG59250.013.1441913
TACATGG242850.013.0631762
ATAACGC5900.013.0507353
Produced by FastQC (version 0.11.5)