FastQCFastQC Report
Wed 25 May 2016
SRR1699993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7600166
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN291380.3833863628768109No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN137220.18054868801549862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN102870.13535230677856247No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN100600.13236552991079406No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN89580.1178658466144029No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN81740.10755028245435691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA6950.016.46124518
TCGCGCA7150.016.30849817
AATCGCG7400.015.75732715
GGACCGT3650.015.6705626
GTACCTG53150.015.49861051
CGCAACG7700.015.14250920
TACCTGG52500.014.7501372
ACGATCG2900.014.41346220
GCGCAAC7850.014.29369819
AACGATA8600.013.81336223
CGACGCT4950.013.7773721
ATAACGC4550.013.538153
CGCGAAA3151.8189894E-1213.26927425
ACCTGGG56400.013.2621053
GCAATCG9000.013.20039113
ATCGCGA3001.2732926E-1113.19943523
ACGATAA9650.012.99426324
GCAACGA9000.012.95517321
TATAACG4100.012.8777522
GAGTACT144700.012.72509612
Produced by FastQC (version 0.11.5)