FastQCFastQC Report
Wed 25 May 2016
SRR1699992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6615532
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN125590.1898411193536665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN114720.17341009007287697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN87680.13253658209196176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG66200.024.6248232
GTACCTG68800.024.0738091
ACCTGGG71850.022.3203243
ATCGCGA2000.020.89921423
ACGATCG2050.019.31795120
CCTGGGG57900.017.5154844
CGCGAAA2300.017.21674325
CGCGCAA4750.017.13736218
TCGCGCA5150.016.23399417
TACCCGG6200.015.6125532
AATCGCG5600.015.32154715
TCGCGAA2601.8189894E-1215.23019524
CGATCGC2900.015.17299121
GCGCAAC5400.015.0747619
GTATCAA217200.014.9368281
CGCAACG5450.014.9364620
ATAACGC4750.014.820273
GTACCCG6650.014.5729151
GCAACGA5850.014.291141521
TAACGCA4850.014.0611134
Produced by FastQC (version 0.11.5)