FastQCFastQC Report
Wed 25 May 2016
SRR1699991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13967205
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN877120.6279853413764601No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN443230.31733621723172245No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN293930.2104429626399842No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN285910.20470094052460747No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN238170.17052087371811325No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN202690.14511851154185823No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN192240.1376366996832938No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN177320.12695453385269279No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN176790.126575073538335No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGN156230.111854877192681No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAN144600.10352822916252752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG103500.018.9210591
TACCTGG102850.018.2030032
ATCGCGA5150.017.513823
ACGATCG4900.016.61235420
CGCGAAA5000.016.27929125
TCGCGAA5350.016.03668424
CGCGCAA11400.016.01759318
TCGCGCA11450.015.94804717
ACCTGGG115450.015.6441323
AATCGCG11800.015.47484415
TATAACG7650.014.3789222
CGCAACG13000.014.04624720
TAGACCG3001.8189894E-1213.9326785
TAACGCA7750.013.9090224
GCGCAAC13700.013.32855519
AGCGTCG6150.013.23538540
GCAACGA14150.013.05992321
ATAACGC8600.013.04589753
GCAATCG14800.012.93214713
AACGATA15000.012.90610523
Produced by FastQC (version 0.11.5)