FastQCFastQC Report
Wed 25 May 2016
SRR1699990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523089
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN137800.24949806168251135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN73350.1328061162874616No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN68610.12422396235150293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG57950.022.3152031
TACCTGG57000.022.1921462
ACCTGGG59900.020.7504713
CCTGGGG50400.016.455734
GTACCGG5000.014.9804581
CGCGCAA3400.014.881927518
TATAACG3750.014.6661142
TAACGCA4100.014.4872594
AATCGCG3500.014.45712215
GTACACG5500.014.4196921
GTATCAA186200.013.6652781
TCGCGCA4300.013.30230617
GTATTAT13350.013.2015491
GTATAAC8850.013.1931581
GCGCAAC3950.012.8097619
CGCAACG3950.012.8097620
CGATCGT3102.3646862E-1112.7740614
ATAACGC4650.012.7737133
GTACATG155950.012.7561191
ACGATCG1751.6000571E-512.5710720
Produced by FastQC (version 0.11.5)