FastQCFastQC Report
Wed 25 May 2016
SRR1699989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5699210
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN163930.28763635661784703No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN82180.14419542357625006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN80710.14161611872522684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG60100.023.3901982
GTACCTG61800.023.311011
ACCTGGG63700.021.619343
TATAACG4000.021.4492512
TAACGCA4150.019.083674
ATAACGC4500.018.0882593
CGCGGTA2250.017.59923243
CCTGGGG50800.015.7199244
GTATTAG16400.015.04341
TTAACGG2701.8189894E-1214.66602735
TATACCG2305.966285E-1014.34725
CGCGAAA1554.5735014E-614.19355125
TCTATAC13700.013.8097373
GTATAGA16750.013.2824561
GTATCAA207950.012.79616451
GTACATG150950.012.7395151
TACATGG151000.012.2234152
ACGATCG1802.1359636E-512.22222520
GTACCCG6700.012.1646241
AGAGTAC188050.012.14347511
Produced by FastQC (version 0.11.5)