FastQCFastQC Report
Wed 25 May 2016
SRR1699988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6350865
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN122590.19302882363268625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN103020.1622141235878892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN72620.1143466283726705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG60950.024.5758481
TACCTGG61550.023.4825952
ACCTGGG65050.021.4411133
TATAACG3950.017.265272
TAACGCA4600.017.2167034
CCTGGGG51500.016.5740974
TAGACCG1351.0876811E-616.2956455
ATAACGC4400.015.9993613
GTCGTAC1704.4305125E-815.5491131
GTACCCG6200.014.9221331
GTATCAA193400.014.43090251
GTACATG152400.014.092661
CGACACG2052.9353032E-813.95066336
GTACCGG4600.013.887161
TACATGG150900.013.383272
TACCCGG6950.013.29453852
CGCGCAA3500.013.19957718
GTATTAG17400.013.0395111
GGTATCA131700.013.0126911
ACATGGG151450.012.8406893
Produced by FastQC (version 0.11.5)