FastQCFastQC Report
Wed 25 May 2016
SRR1699987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6896588
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN127600.18501902679991905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN95270.138140773379532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA5450.017.35908718
GTACCTG50450.016.725241
TACCTGG48400.016.6369972
TCGCGCA6150.016.09875717
GCGCAAC6400.015.12579519
GTATCAA200700.014.9617791
AATCGCG6450.014.66616215
CCTATAC11300.014.600953
GTACATG185800.013.9205741
ATCGCGC7500.013.49423816
AACGATA7050.013.41778323
TACATGG185350.013.3654942
ACCTGGG57750.013.2563973
GGTATCA133500.012.9380721
CGCAACG7550.012.82186620
GCAACGA7400.012.78445521
ACATGGG189550.012.7431513
GCAATCG7800.012.69187113
ATCAACG235350.012.5907523
TCAACGC235800.012.5480654
Produced by FastQC (version 0.11.5)