FastQCFastQC Report
Wed 25 May 2016
SRR1699986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5557082
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN197610.35560029526287357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN103500.18624882627249337No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN93200.16771391892363652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN75730.13627655665329394No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN66620.11988306093017882No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN60550.10896006213332823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG41750.017.2061941
TACCTGG42200.016.4744152
ACCTGGG45350.014.8437743
CGCGCAA5050.014.81218118
CGCGAAA2358.240022E-1014.04195425
ATCGCGA2104.0259692E-813.618710523
GTACATG172450.013.4551611
TATACTG17850.013.4334795
TATAACG3151.8189894E-1213.2702292
TACATGG170700.013.1333672
AATCGCG5550.013.08040315
CGACGCT3900.012.97462221
GGACCGT2756.2937033E-1012.7993386
AACGATA5700.012.736526523
TCGCGCA5750.012.62634317
TACCGAC4200.012.57168318
GAGTACT124500.012.47485712
ACATGGG172000.012.4574963
GTATATA23350.012.4569231
GTATCAA240650.012.3523561
Produced by FastQC (version 0.11.5)