FastQCFastQC Report
Wed 25 May 2016
SRR1699985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4032701
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN95220.2361196627272887No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN77350.19180693039231028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN67210.16666249245852843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN45900.11381949715587641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG56550.027.8557592
GTACCTG57650.027.8146171
ACCTGGG60850.025.4172943
TAACGCA3350.024.9543574
TATAACG3550.023.549942
ATAACGC4400.023.0006283
CCTGGGG44650.021.5311684
TCGCGCA2400.019.2498117
CGCGCAA2250.018.57759518
AATCGCG2500.018.47981815
GTACCGG4250.016.5844881
GTATAAC7850.015.9935991
GTACCCG4750.015.7661741
CTGGGGG24300.015.4809455
GTATCAA138300.015.2734611
TACCCGG4850.014.5158612
GCAATCG3250.014.21524413
GGTATCA91650.014.1073891
GTACATG101100.013.6166371
TATCACG4600.013.391672
Produced by FastQC (version 0.11.5)