FastQCFastQC Report
Wed 25 May 2016
SRR1699984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7515771
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN219690.2923053403303533No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN110610.1471705298099157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN97820.13015298097826558No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN85630.11393375343660683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA12300.020.03346618
CGCAACG12750.019.67125320
TCGCGCA12800.019.4227917
AATCGCG12800.019.4211115
GCGCAAC13500.018.74137519
AACGATA14000.018.54200623
GCAACGA13900.018.04377721
ATCGCGA5600.016.8920823
CGCGAAA5600.016.49913225
TCGCGAA5800.015.93019624
ATCGCGC15650.015.88562816
TACCGAC10300.015.80657118
CGCGGTA5600.015.71345943
TAACGGC6300.015.36427136
ACCGACG10750.015.14469819
GCTACCG10800.015.07468416
ACGATCG6000.015.03384320
CGACGCT10750.014.9400421
GCAATCG16800.014.79703713
CCGACGC11200.014.53620720
Produced by FastQC (version 0.11.5)