FastQCFastQC Report
Wed 25 May 2016
SRR1699983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5570789
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN66420.11922907150136183No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN59490.10678918192737151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG59400.024.8780421
TACCTGG59850.024.1138972
ACCTGGG64700.022.0336673
GTACACG4750.018.0821931
TATAACG3850.017.7144932
CCTGGGG51250.016.9555344
GTATCAA135450.016.8771271
GGTATCA81950.016.204981
ATAACGC4000.015.9497553
CGCGAAA1351.9590982E-514.66617825
CGATCGT3550.014.25304741
TACACGG5250.014.2477852
GTACCCG6650.014.2405571
ATCAACG159250.014.0079483
CCGATCG950.006244939313.8942769
TCAACGC160550.013.8668694
TATCAAC164400.013.7167042
CGACGCT3400.013.58790621
GTACATG145700.013.4829661
TAACGCA4250.013.4583764
Produced by FastQC (version 0.11.5)