FastQCFastQC Report
Wed 25 May 2016
SRR1699982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4194245
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN175460.41833512348467955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN92370.2202303394293848No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN81640.19464766602809325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN65320.15573720657710743No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN54440.12979690027645024No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN53780.12822331551924127No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN47070.1122252038209499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN44750.1066938149774274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG56900.030.006371
TACCTGG57250.029.0892872
TATAACG3900.028.2044772
ACCTGGG61900.026.40653
TAACGCA4350.025.7918954
ATAACGC4550.024.6587733
CCTGGGG42450.021.7139424
GTACCGG3600.020.806571
CTGGGGG21100.017.3072455
TACCCGG5750.017.2169972
GTACACG3850.017.166611
GTACCCG5850.016.9465261
CGTATCG800.001990076416.49922810
GTATAAC10200.015.9829011
CGCCGAC1351.9575773E-514.66720418
ACACGGG3900.014.6663283
GTATTAG12350.014.270761
GTCTCGC5300.014.1327631
TACACGG4400.013.9996782
CGATCGT950.006241713713.89524514
Produced by FastQC (version 0.11.5)