FastQCFastQC Report
Wed 25 May 2016
SRR1699981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12784218
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA215350.017.1244891
GTACCTG83500.016.7003021
GGTATCA129450.016.6945231
TACCTGG81750.016.3077342
ACGATCG4000.015.95023720
CGAACTA7650.015.52854929
CGCGCAA13550.015.26204118
AATCGCG13700.015.09529215
ATCGCGA4400.014.99934223
CGATCGT14100.014.9783641
CGCGAAA4200.014.66602425
TCAACGC245250.014.5133644
ATCAACG245450.014.4925753
TCGCGCA14550.014.36481217
CGCAACG14250.014.35810820
GTACATG356550.014.1303451
GCGCAAC14900.014.02705519
TCGCGAT14900.014.02650538
CAACGCA255750.013.9261085
TCGCGAA4600.013.86895724
Produced by FastQC (version 0.11.5)