FastQCFastQC Report
Wed 25 May 2016
SRR1699980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10812671
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN564960.5224980950590284No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN280510.2594271110255736No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN185130.17121578932716994No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN169810.15704722727622064No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN145750.13479555606565669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN137640.127295096650957No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN121470.11234041986480492No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN115600.10691160398758087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG122950.026.5321561
TACCTGG122150.026.1325262
ACCTGGG134000.023.1809543
TATAACG7250.022.757822
ATAACGC8800.019.2490583
CCTGGGG95450.018.7607364
GTACCGG10350.018.7278691
TAACGCA8350.018.7056714
GTACACG8650.014.7692311
TACCCGG11200.014.3387822
ATCGCGA3051.8189894E-1213.70443723
GTACCCG12900.013.6598611
CGCGAAA3300.013.33286525
TCGCGCA6450.013.30247317
GTACATG259900.013.25491
CTGGGGG55300.013.1277485
GTATAAC21100.012.9444991
GTACGCA6200.012.7735673
TATACTG31350.012.7713045
TACACGG9350.012.7054362
Produced by FastQC (version 0.11.5)