FastQCFastQC Report
Wed 25 May 2016
SRR1699979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8805505
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN406720.46189287269725016No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN211470.24015658386429853No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN143640.16312522677574995No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN134750.15302926975795256No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN106540.12099249276446951No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN93390.1060586530812259No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN92020.10450280818646973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG74300.020.8734281
TACCTGG75400.019.810882
ACCTGGG82150.017.7278393
CGCGCAA7050.016.8506818
AATCGCG6750.016.29620215
CGCGAAA2101.5097612E-1015.71374825
TACGCGA850.002982723315.52879144
GCGCAAC7500.015.25298519
ACGATCG2355.0931703E-1114.97838220
GTATTAG22100.014.3542391
CGCAACG8000.013.74968620
TCGCGCA8300.013.51799417
ATACCGA2805.820766E-1113.356616
ATCGCGA2653.4924597E-1013.28271523
GTACATG248150.013.2808931
GTCGTAC3650.013.2782171
AACGATA8150.013.22669223
TATACCG4000.013.1994735
GTATAGA20050.013.18491
CCTGGGG73600.012.8826394
Produced by FastQC (version 0.11.5)