FastQCFastQC Report
Wed 25 May 2016
SRR1699978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9868224
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGA2950.018.6438823
TCGCGAA2950.017.89812524
CGCGAAA3000.017.59973125
CGCGCAA11950.017.30491318
AATCGCG12450.016.96359815
CGCAACG12950.016.47859620
TCGCGCA12700.016.28321817
GGTATCA126100.016.1573891
GTATCAA202400.016.1389471
AACGATA14050.015.65820523
GCGCAAC13750.015.51976319
GTACCCG7700.015.1610641
GTACCTG59450.015.1165551
GCAACGA14400.014.97207221
CGAACTA6650.014.8866929
ACGATCG3600.014.66651920
TACCTGG60600.014.5935422
TAACGCA3650.014.46524054
CGCGGTA5350.014.39201343
CGATCGT11700.014.10206341
Produced by FastQC (version 0.11.5)