FastQCFastQC Report
Wed 25 May 2016
SRR1699977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8971609
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN282790.31520544419624175No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN138680.15457650907434775No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN94840.1057112497880815No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN92970.10362689680301493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG74350.021.037542
GTACCTG77650.020.9346731
CGCGAAA1901.8189894E-1218.52623725
ACCTGGG83100.018.2929333
AATCGCG6750.017.92565215
CGCGCAA7150.016.30716918
CGCAACG7750.016.18066820
TCGCGCA7400.016.05380817
AACGATA7650.015.81692723
GCGCAAC7250.015.77915719
ACGATCG2101.5097612E-1015.71430820
ATCGCGA2355.0931703E-1114.97866123
CGATCGT5750.014.92117841
CGACGCT6000.014.66668721
TCGCGAT6350.014.55063538
GCAACGA8050.014.48449221
TTCGCGA6750.014.34020137
CCTGGGG74150.014.1518614
TTTCGCG6100.014.06504436
ATCGCGC8300.014.04797716
Produced by FastQC (version 0.11.5)