FastQCFastQC Report
Wed 25 May 2016
SRR1699976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6475470
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG60150.021.3930551
TACCTGG60150.020.884862
ACCTGGG63500.019.0541613
GTATCAA138350.017.4075071
GGTATCA85650.016.5159381
TCAACGC162900.014.3553264
ATCAACG163450.014.3339393
CGCGGTA3250.014.21467143
CAACGCA168400.013.9387315
TATCAAC170350.013.909282
TAACGCA4000.013.749314
CCTGGGG54000.013.6882024
AACGCAG173150.013.5944666
CGAACTA3400.013.58755329
GTATAGG13750.013.460841
GTACATG172000.013.3613841
TATACTG19600.013.3564725
GTACCGG5650.013.2594671
TACATGG171650.012.8939282
GTACTAG7350.012.8907051
Produced by FastQC (version 0.11.5)