FastQCFastQC Report
Wed 25 May 2016
SRR1699975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5902154
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55500.022.6650091
TACCTGG51200.020.883392
GTACCTG52250.020.6174681
GTATCAA102600.020.5054241
CGCGCAA4350.019.21843518
TCGCGCA5000.018.48004317
ACCTGGG56250.018.4597053
GCGCAAC5200.016.49989919
TCAACGC125300.016.3457154
ATCAACG125350.016.3040943
AACGATA5150.016.23235923
ACGATCG1501.8412538E-716.13323220
ATCGCGC5350.016.0374216
CAACGCA131600.015.5799235
TATCAAC132600.015.5464342
CGCAACG5400.015.48138520
AATCGCG5450.015.33896515
AACGCAG136900.015.1213826
GCAACGA5500.014.7999121
CGCGAAA1655.429665E-714.666079525
Produced by FastQC (version 0.11.5)