FastQCFastQC Report
Wed 25 May 2016
SRR1699974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8107176
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN282210.34809901746304756No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN138370.17067595424103288No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN101220.12485235302650392No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN89440.11032201595228722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA11600.017.63877518
ACGATCG3450.017.21715520
ATCGCGA3600.017.11034823
TCGCGCA12250.016.88244217
AATCGCG12450.016.43431915
CGCGAAA3800.016.20980325
GCGCAAC12600.016.06416319
CGCAACG12700.015.93678920
CGACGCT11500.015.87784821
AACGATA13050.015.50888823
CGATCGT11250.015.25265341
TACCTGG52500.015.1274112
GTACCTG55000.015.0984171
ATCGCGC13750.014.880712516
GCAACGA13900.014.8773121
GCAATCG14450.014.46391413
TCGCGAA4450.014.3364424
TTTCGCG12500.014.25536536
TCGCGAT12450.014.13591638
ACCGACG12700.014.03208219
Produced by FastQC (version 0.11.5)