FastQCFastQC Report
Wed 25 May 2016
SRR1699973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7173555
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN290620.40512688618125875No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN145180.20238222192483363No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN98240.13694744098288783No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN93830.13079986143550862No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN72460.10100988979662105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG62500.020.5531351
TACCTGG61400.020.4243222
ACCTGGG65900.018.4270363
AATCGCG5400.017.9251915
CGCGCAA5700.017.36867518
TCGCGCA6250.015.84023317
GCGCAAC6350.015.24421319
ATCGCGC6400.015.12522416
GCAACGA6550.015.11462121
AACGATA6800.014.558125523
CGCAACG6700.014.44787320
GTACATG185500.013.790431
GCAATCG7350.013.46883513
TACATGG184300.013.3342632
CGATCGT5950.013.31028641
TATACTG20400.013.264075
CCTGGGG58500.013.1617624
CAATCGC7550.013.11204514
GTATTAA21300.013.0340821
TATAACG3900.012.9749122
Produced by FastQC (version 0.11.5)