FastQCFastQC Report
Wed 25 May 2016
SRR1699972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5399504
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG41650.020.1770172
GTACCTG43550.019.9311521
GTATCAA102500.019.7092381
GGTATCA55950.019.333291
ACCTGGG45250.017.745283
CGCGCAA3500.016.97166418
AATCGCG3650.016.27419915
TCAACGC123850.015.8798384
ATCAACG123850.015.8089333
GCGCAAC3800.015.63179619
CAACGCA127150.015.4503985
CCGCGTA1004.9492077E-415.3993599
AACGCAG131500.015.0229486
TATCAAC132000.014.9661832
TCGCGCA4150.014.84357917
GCGAACT2950.014.168900528
CGCAACG4350.013.65485720
GTACATG136300.013.3993291
GTATAGG11400.013.334251
ACCGCGT2502.0809239E-913.19945058
Produced by FastQC (version 0.11.5)