FastQCFastQC Report
Wed 25 May 2016
SRR1699971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6722102
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN618780.9205156363292315No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN302830.45049896594844885No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN218200.32460084658042976No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN200140.29773425038775075No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN168430.25056150590990733No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN149240.22201388791779714No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN141490.21048475610753897No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN125180.1862215122591118No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGN105630.1571383474990412No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAN98000.1457877312781032No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAN95690.14235130618369074No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCN89060.13248831987375378No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCN68690.10218529858666234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG48600.017.8136861
TACCTGG48250.017.7818322
CGCGAAA1408.393181E-817.2849225
ATCGCGA1602.1353117E-816.4992423
CGAACTA3750.015.83927229
ACCTGGG53300.015.643043
ACGATCG1554.5756333E-614.19300120
TACGCAG5150.014.0964394
AGTACGC5700.013.5083512
GTACGCA5800.012.8961523
GTACATG177800.012.8482661
GTATATA25050.012.7513971
GTCTACG4350.012.6604241
CGCGCAA4900.012.57197418
GTATTAA18800.012.4206831
CCGATCG1953.556559E-612.4096869
TACATGG176800.012.3186222
GAGTACT101850.012.2469512
GCGAACT4500.012.22166228
GTATTAG17850.012.2178411
Produced by FastQC (version 0.11.5)