FastQCFastQC Report
Wed 25 May 2016
SRR1699970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3279871
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN69030.2104655945310044No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN36860.11238246870075073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG54050.030.3230192
GTACCTG54400.030.128361
ACCTGGG59000.027.2561153
CCTGGGG41750.023.078964
TACCCGG4350.020.2293822
GTACCCG4500.020.0711781
TATAACG2000.019.7995052
ATAACGC2150.019.4407833
CTGGGGG21350.017.5165945
TACCGTC1307.3304545E-716.9221387
TCTGTCG7350.016.7609758
GTACACG3450.016.6018221
ACGTCCG1351.0874628E-616.29539343
TAACGCA2201.8189894E-1115.9991134
TACACGG3500.015.0853382
CGCGACT2950.014.91488233
CTAGCGT900.00436273114.6658534
TCGCTCT7200.014.0547754
TCTCGCT6800.013.9114172
TACACCG2703.274181E-1113.8510855
Produced by FastQC (version 0.11.5)