FastQCFastQC Report
Wed 25 May 2016
SRR1699969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10941127
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN214720.19625034971260275No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN111930.10230207546261003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA12450.016.78719918
ATCGCGC13100.016.62623216
AATCGCG12900.016.54268515
AACGATA13400.016.25311323
TCGCGCA13800.015.94229617
CGCAACG13550.015.74926920
CGCGAAA4650.015.612308525
GCGCAAC13600.015.529619
ACGATCG4300.015.34902420
TATACCG3450.014.6659745
GCAACGA14600.014.61628221
GTACCTG73450.014.365471
CGCGTAA1707.6105425E-714.23462110
TACCTGG71350.013.967362
GTACATG294700.013.708671
ACGATAA16600.013.65008324
GCAATCG15800.013.64511113
TACATGG290200.013.4482662
CAATCGC16150.013.34970214
CGGCTAT6400.013.06188330
Produced by FastQC (version 0.11.5)